G9a, active human recombinant protein (expressed E. coli)

Item number Size Datasheet Manual SDS Delivery time Quantity Price
BPS-51000 50 µg - -

3 - 15 business days*

533.00€
 
Active human G9a (euchromatic histone-lysine N-methyltransferase 2) (GenBank Accession No.... more
Product information "G9a, active human recombinant protein (expressed E. coli)"
Active human G9a (euchromatic histone-lysine N-methyltransferase 2) (GenBank Accession No. NM_006709) (amino-acids 785-1210), with N-terminal GST tag, MW = 74.6 kDa, expressed in an E. coli expression system. Assay conditions: 50 µl reaction mix (50 mM TRIS pH8.8, 5 mM MgCl2, 4 mM DTT, 20 µM S-adenosylhomocysteine, and 0-40 ng G9a) add to the wells coated with the substrate. Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Finally, incubate 1h with the Europium-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm).
Keywords: BAT8, EHMT2, Protein G9a, H3-K9-HMTase 3, HLA-B-associated transcript 8, Lysine N-methyltransferase 1C, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase EHMT2, Euchromatic histone-lysine N-methyltransferase 2,
Supplier: BPS Bioscience
Supplier-Nr: 51000

Properties

Application: Enzyme kinetics, inhibitor screening
Conjugate: No
MW: 74.6 kD
Purity: >70%
Format: Solution

Handling & Safety

Storage: -80°C
Shipping: -20°C (International: -20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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