Lipid Droplets Fluorescence Assay Kit
Lipid droplet biogenesis regulator studies
Cayman's Lipid Droplets Fluorescence Assay Kit can be used to study regulators of lipid droplet biogenesis in a variety of conditions, including inflammation. The main advantage of this assay is that the green fluorescence of Nile Red is both very sensitive and specific for lipid droplets. Furthermore, changes in lipid droplet biogenesis in various cell types in response to different manipulations can be both qualified and quantified by fluorescence microscopy, flow cytometry, or fluorescence plate readers. Simultaneous visualization of lipid droplets and associated proteins is also possible when used with an antibody conjugated to a blue fluorophore. Oleic acid, which is commonly used to induce lipid droplet formation in cultured cells, is included as a positive control. This kit provides sufficient reagent to effectively treat/stain 480 individual wells of cells when utilized in a 96-well plate format. Lower density plates will still require approximately the same amount of reagent on a per plate basis. Therefore, in effect 5 plates worth of cells can be examined irrespective of the number of wells/plate. Exceptions include protocols in which non-adherent cells are utilized.
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