The maintenance of embryonic stem cells in culture typically requires feeder cells and various exogenous factors found in serum. Murine embryonic stem (mES) cells can be maintained in the absence of feeder cells and serum but require the leukemia inhibitor factor (LIF) and bone morphogenic protein (BMP) to prevent...

The PtdIn phosphates play an important role in the generation and transduction of intracellular signals. PtdIns-(3,4,5)-P3-biotin is an affinity probe which allows the PIP3 to be detected through an interaction with the biotin ligand. This design allows PtdIns-(3,4,5)-P3-biotin to serve as a general probe for any...

Arachidonic acid is metabolized by cytochrome P450 (CYP450) 4A omega-hydroxylase to 20-HETE, which acts as a vasoconstrictor in the kidney and brain. During mass spectrometry lipidomics analysis of rat brain, a series of fatty acyl amides of taurine was discovered, of which arachidonoyl taurine is a prominent...

KIAA1363 is a recently characterized 2-acetyl monoacylglyceryl ether hydrolase that is critical to the survival and proliferation of many cancer cell lines. 2-thioacetyl MAGE is a colorimetric substrate for KIAA1363. It can readily be adapted to perform activity/inhibition studies of this new enzyme, using standard...