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Cay10007640-480 Cholesterol Fluorometric Assay Kit
Cholesterol quantification Erklärungen der Abkürzungen ansehen.
Cayman's Cholesterol Assay provides a simple fluorometric method for the sensitive quantitation of total cholesterol in plasma or serum. In our assay, cholesteryl esters in the sample are first hydrolyzed by cholesterol esterase. All cholesterol, whether previously esterified or existing free in the circulation, is then oxidized by cholesterol oxidase to the corresponding ketone and hydrogen peroxide. ADHP (10-acetyl-3,7-dihydroxyphenoxazine) is utilized as a highly sensitive and stable probe for hydrogen peroxide. Horseradish peroxidase catalyzes the reaction of ADHP with hydrogen peroxide to yield the highly fluorescent product resorufin which can be monitored using excitation wavelength of 530-580 nm and emission wavelengths of 585-595 nm.
Cayman
Icon Datasheet
480 wells

Buy Bulk

487 €

Cay10007640-96 Cholesterol Fluorometric Assay Kit
Cholesterol quantification Erklärungen der Abkürzungen ansehen.
Cayman's Cholesterol Assay provides a simple fluorometric method for the sensitive quantitation of total cholesterol in plasma or serum. In our assay, cholesteryl esters in the sample are first hydrolyzed by cholesterol esterase. All cholesterol, whether previously esterified or existing free in the circulation, is then oxidized by cholesterol oxidase to the corresponding ketone and hydrogen peroxide. ADHP (10-acetyl-3,7-dihydroxyphenoxazine) is utilized as a highly sensitive and stable probe for hydrogen peroxide. Horseradish peroxidase catalyzes the reaction of ADHP with hydrogen peroxide to yield the highly fluorescent product resorufin which can be monitored using excitation wavelength of 530-580 nm and emission wavelengths of 585-595 nm.
Cayman
Icon Datasheet
96 wells

Buy Bulk

172 €


 

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