PDE7A-HEK293 Human Recombinant Cell line, 1x10(6) cells

PDE7A-HEK293 Human Recombinant Cell line, 1x10(6) cells
Item number Size Datasheet Manual SDS Delivery time Quantity Price
BPS-60407 2 vials (2 vials) - -

3 - 15 business days*

11,632.00€
 
Recombinant HEK293 cell line expressing human PDE7A (phosphodiesterase 7A, accession number... more
Product information "PDE7A-HEK293 Human Recombinant Cell line, 1x10(6) cells"
Recombinant HEK293 cell line expressing human PDE7A (phosphodiesterase 7A, accession number NM_002603). Format: Each vial contains 1 X 106 cells in 1 ml of 10% DMSO. Functional validation: N-terminal FLAG-tagged human PDE7A has been stably expressed in a human embryonic kidney (HEK293) cell line. PDE7A expression was confirmed by Western blotting. The regulation of intracellular level of cAMP by PDE7A in PDE7A stably-expressed HEK293cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. When cells transiently transfected with pCRE-luc reporter were activated by forskolin, the level of cAMP was upregulated in parental HEK293 cells inducing the expression of the luciferase reporter whereas hPDE7A-HEK293 cells showed reduction in the level of cAMP that resulted in lowered expression of luciferase. Inhibition of PDE7A activity by BRL 50481, a PDE7A inhibitor, restored the cAMP level, resulting in higher luciferase activity. Storage: Store cells in liquid nitrogen upon arrival. Avoid freeze/thaw cycles. References: Malik, R. et al. (2008) Appl. Microbiol. Biotechnol. 77 (5): 1167-1173. Fan Chung, K. (2006). Eur. J. Pharmacol. 533(1-3):110-117.
Keywords: HCP1, TM22, High affinity cAMP-specific 3',5'-cyclic phosphodiesterase 7A,
Supplier: BPS Bioscience
Supplier-Nr: 60407

Properties

Application: Cellular assays

Handling & Safety

Storage: Liquid nitrogen
Shipping: -80°C (International: -80°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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