PDE1B assay kit

The PDE1B Assay Kit is designed for identification of PDE1B inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE1B to the binding agent. PDE1B is a calcium-dependent cyclic nucleotide phosphodiesterase that is highly expressed in the striatum. It plays the physiological role in the central nervous system. The PDE1B inhibitor screening assay kit comes in a convenient 96-well format, with purified PDE1B enzyme, fluorescently labeled PDE1B substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE1B Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE1B reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE1B for 1h. Second, binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.