||SUV39H1 chemiluminescent assay kit||
SUV39H1 chemiluminescent assay kit
Enzyme kinetics, HTS
The SUV39H1 Direct Activity Assay kit is designed to measure SUV39H1 activity using purified SUV39H1 or SUV39H1 extracts. The SUV39H1 Direct Activity Assay Kit comes in a convenient format, with a 96-well plate precoated with histone H3 peptide substrate, the antibody against methylated lysine residue of Histone H3, the secondary Europium-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified SUV39H1 enzymes for 100 enzyme reactions. The key to the SUV39H1 Direct Activity Assay Kit is a highly specific antibody that recognizes methylated K9 residue of Histone H3. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme for one hour. Next, primary antibody is added. Finally, the plates are treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
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