Enzyme kinetics, inhibitor screening Active human G9a (euchromatic histone-lysine N-methyltransferase 2) (GenBank Accession No. NM_006709) (amino-acids 785-1210), with N-terminal GST tag, MW = 74.6 kDa, expressed in an E. coli expression system. Specific Activity: 146 pmol/min/mg Assay conditions: 50 µl reaction mix (50 mM TRIS pH8.8, 5 mM MgCl2, 4 mM DTT, 20 µM S-adenosylhomocysteine, and 0-40 ng G9a) add to the wells coated with the substrate. Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Finally, incubate 1h with the Europium-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm).MW : 74 kD Purity: >70%UniProt ID: Q96KQ7
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- Geninformation Weitere Produkte für dieses Protein: Antibodies
Anti-EHMT2 (M), Kat #: AC-R0438-3
Anti-EHMT2 (N-term), Kat #: WA-AP5526a.20
Anti-EHMT2 (N-term), Kat #: WA-AP5526a
Anti-G9a (EHMT2, BAT8, C6orf30, Em:AF134726.3, Euchromatic histone-lysine N-methyltransferase 2, G9A, H3-K9-HMTase 3, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase, H3 lysine-9 specific 3, HLA-B-associated transcript 8, NG36, NG36/, Kat #: G1009-02A.100
Anti-G9a, ID (Histone-lysine N-methyltransferase, H3 Lysine-9 Specific 3, Histone H3-K9 Methyltransferase 3, H3-K9-HMTase 3, Euchromatic Histone-lysine N-methyltransferase 2, HLA-B Associated Transcript 8, G9a Protein, EHMT2, Lysine N-methyltransferase 1C, Kat #: G1009-02D.100
Anti-G9a, NT (Histone-lysine N-methyltransferase, H3 Lysine-9 Specific 3, Histone H3-K9 Methyltransferase 3, H3-K9-HMTase 3, Euchromatic Histone-lysine N-methyltransferase 2, HLA-B Associated Transcript 8, G9a Protein, EHMT2, Lysine N-methyltransferase 1C, Kat #: G1009-02C.100
Anti-G9A/EHMT2, Kat #: A300-933A
Anti-G9A/EHMT2 (IHC), Kat #: IHC-00489
Anti-KMT1C / G9a / BAT8 (Center), Kat #: WA-AP1197c.20
Anti-KMT1C / G9a / BAT8 (Center), Kat #: WA-AP1197c
Enzymes & Proteins
G9a (expressed Sf9), active human recombinant protein, Kat #: BPS-51001
G9a (human recombinant), Kat #: Cay10353-50
G9a (human recombinant), Kat #: Cay10353-100
G9a (human recombinant), Kat #: Cay10353-25
G9a, active, Recombinant, Human (SET Domain-containing Protein, G9a Histone Methyltransferase, G9a HMTases), Kat #: G1009-01.50
siRNA
ELISA and Assay Kits
Primers
Peptides
Relevant Pathways: Products for: EHMT - euchromatic histone-lysine N-methyltransferase [EC:2.1.1.43]
Schlagworte: Methyltransferase BAT8, EHMT2, EC=2.1.1.-, Protein G9a, EC=2.1.1.43, H3-K9-HMTase 3, Lysine N-methyltransferase 1C, HLA-B-associated transcript 8, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase EHMT2