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G9a, active human recombinant protein (expressed E. coli)

ART NR.: BPS-51000 (50 µg)

Hersteller: BPS


Anwendungen: Enzyme kinetics, inhibitor screening

MW: 74 kD

Reinheit: >70%

Format: solution

Lagerung: -80°C

Versand: -20°C

UniProt ID: Q96KQ7 | Suche mit UniProt ID

Gene ID: Gene ID NM_006709 | Suche mit Gene ID

KEGG ID: K11420 | Suche mit KEGG ID

Schlagworte: Methyltransferase BAT8, EHMT2, EC=2.1.1.-, Protein G9a, EC=, H3-K9-HMTase 3, Lysine N-methyltransferase 1C, HLA-B-associated transcript 8, Histone H3-K9 methyltransferase 3, Histone-lysine N-methyltransferase EHMT2


50 µg

510 €

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Bemerkungen: Active human G9a (euchromatic histone-lysine N-methyltransferase 2) (GenBank Accession No. NM_006709) (amino-acids 785-1210), with N-terminal GST tag, MW = 74.6 kDa, expressed in an E. coli expression system. Assay conditions: 50 µl reaction mix (50 mM TRIS pH8.8, 5 mM MgCl2, 4 mM DTT, 20 µM S-adenosylhomocysteine, and 0-40 ng G9a) add to the wells coated with the substrate. Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Finally, incubate 1h with the Europium-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm).


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