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BPS-51000
G9a, active human recombinant protein (expressed E. coli)
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50 µg

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440 €

G9a, active human recombinant protein (expressed E. coli)


Enzyme kinetics, inhibitor screening Erklärungen der Abkürzungen ansehen.

Active human G9a (euchromatic histone-lysine N-methyltransferase 2) (GenBank Accession No. NM_006709) (amino-acids 785-1210), with N-terminal GST tag, MW = 74.6 kDa, expressed in an E. coli expression system. Specific Activity: 146 pmol/min/mg Assay conditions: 50 µl reaction mix (50 mM TRIS pH8.8, 5 mM MgCl2, 4 mM DTT, 20 µM S-adenosylhomocysteine, and 0-40 ng G9a) add to the wells coated with the substrate. Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Finally, incubate 1h with the Europium-labeled secondary antibody. Time resolved fluorescence measured at room temperature (Lambda exc=330 nm, Lambda em=620 nm).

MW: 74 kD
Purity: >70%

UniProt ID: Q96KQ7

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Products for: EHMT - euchromatic histone-lysine N-methyltransferase [EC:2.1.1.43]



Schlagworte:
Methyltransferase BAT8, EHMT2, EC=2.1.1.-​, Protein G9a, EC=2.1.1.43, H3-​K9-​HMTase 3, Lysine N-​methyltransferase 1C, HLA-​B-​associated transcript 8, Histone H3-​K9 methyltransferase 3, Histone-​lysine N-​methyltransferase EHMT2

 

 

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