Kontakt 0800-2466651 DE | EN Merkliste Login Warenkorb

Reset
Suche
zurück

Fluorogenic SIRT1 (Sir2) Assay Kit

ART NR.: BPS-50081 (96 rxns)

Hersteller: BPS

Datenblatt



Manual

Anwendungen: Enzyme kinetics, inhibitor screening, HTS

UniProt ID: Q96EB6
Suche mit UniProt ID

Geninformation: NM_012238

Schlagworte: EC=3.5.1.-, Sirt1, Sir2, Sir2a, SIR2L1, hSIRT1, NAD-dependent deacetylase sirtuin-1, hSIR2, SIR2-like protein 1 SIRT1, hSIR2, hSIRT1, SIR2L1, EC=3.5.1.-, SIR2-like protein 1, NAD-dependent deacetylase sirtuin-1

96 rxns

429 €

In Warenkorb aufnehmen


Bemerkungen: The Fluorogenic SIRT1 Assay Kit is a complete assay system designed to measure Sirtuin 1 (SIRT1) activity for screening and profiling Applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT1 (Sir2) activity measurements. In addition, the kit includes purified SIRT1 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT1 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT1 activity level. First, the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylated lysine side chain, is incubated with a sample containing SIRT1 activity (e.g. HeLa nuclear extract or purified SIRT1 enzyme). The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader. Components: SIRT1 human recombinant enzyme, 50 µg, Fluorogenic SIRT substrate 1, 50 µl at 5 mM, Nicotinamide Adenine Dinucleotide (NAD), 50 µl at 50 mM, Nicotinamide, 500 µl at 10 mM, 2x SIRT Developer (contains 2 mM Nicotinamide), 6 ml, SIRT assay buffer, 10 ml, 1 black, low binding NUNC black microtiter plate. Applications: Great for studying enzyme kine

Spezifikationen

bps_datasheet_parse.php?nr=BPS-50081&ak=
Loading Loading ...

Verwandte Produkte

    Products for: SIRT1, SIR2L1 - NAD-dependent deacetylase sirtuin 1 [EC:3.5.1.-]

    Relevant Pathways:

    Die Preise verstehen sich exkl. Versandkosten, eventuell anfallender Kühlmittelpauschale sowie der jeweils geltenden Mehrwertsteuer.





    alle Treffer anzeigen (484 INFOS)

     


    Forschungsprodukte im Fokus

     

    Produkte

    Service

    Company

    € 10 Gutschein sichern