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Fluorogenic SIRT1 (Sir2) Assay Kit

ART NR.: BPS-50081 (96 rxns)

Hersteller: BPS



Anwendungen: Enzyme kinetics, inhibitor screening, HTS

Lagerung: Various

Versand: -80°C

UniProt ID: Q96EB6 | Suche mit UniProt ID

Gene ID: Gene ID NM_012238 | Suche mit Gene ID

KEGG ID: K11411 | Suche mit KEGG ID

Schlagworte: EC=3.5.1.-, Sirt1, Sir2, Sir2a, SIR2L1, hSIRT1, NAD-dependent deacetylase sirtuin-1, hSIR2, SIR2-like protein 1 SIRT1, hSIR2, hSIRT1, SIR2L1, EC=3.5.1.-, SIR2-like protein 1, NAD-dependent deacetylase sirtuin-1


96 rxns

510 €

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Bemerkungen: The Fluorogenic SIRT1 Assay Kit is a complete assay system designed to measure Sirtuin 1 (SIRT1) activity for screening and profiling Applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent SIRT1 (Sir2) activity measurements. In addition, the kit includes purified SIRT1 enzyme and a SIRT inhibitor, Nicotinamide, for use as a positive and negative control, respectively. The Fluorogenic SIRT1 Assay Kit is based on a unique fluorogenic substrate and developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the SIRT1 activity level. First, the SIRT fluorometric substrate (SIRT substrate 1), containing an acetylated lysine side chain, is incubated with a sample containing SIRT1 activity (e.g. HeLa nuclear extract or purified SIRT1 enzyme). The deacetylation sensitizes the substrate so subsequent treatment with the Lysine Developer produces a fluorophore that can then be measured using a fluorescence reader. Components: SIRT1 human recombinant enzyme, 50 µg, Fluorogenic SIRT substrate 1, 50 µl at 5 mM, Nicotinamide Adenine Dinucleotide (NAD), 50 µl at 50 mM, Nicotinamide, 500 µl at 10 mM, 2x SIRT Developer (contains 2 mM Nicotinamide), 6 ml, SIRT assay buffer, 10 ml, 1 black, low binding NUNC black microtiter plate. Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.


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