Fluorogenic HDAC class 2a assay kit
The HDAC Class2a Fluorescent Activity Assay kit is a complete assay system designed to measure histone deacetylase, HDAC, class2a activity in cell extracts, nuclear extracts, immunoprecipitates, and purified enzymes. It comes in a convenient 96- well format, with all the reagents necessary for 100 fluorescent HDAC activity measurements. In addition, the kit includes purified HDAC4 and a potent HDAC inhibitor, TrichostatinA, for the positive and negative control. The HDAC Class 2a Fluorescent Activity Assay is based on the unique fluorogenic substrate and Developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC class2a activity level. First, the HDAC fluorometric substrate, is incubated with a sample containing HDAC activity, e.g. purified HDAC4 enzymes. The deacetylation will sensitize the substrate, causing further treatment with the Lysine Developer to produce a fluorophore that can then be measured using a fluorescence reader.
Datenbank vom 22.05.2013, 17:22 Uhr
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