Fluorogenic HDAC Assay Kit
ART NR.: BPS-50033 (100 assays)
Anwendungen: HDAC assay
UniProt ID: Q92769
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Schlagworte: EC=126.96.36.199, Histone deacetylase, HDAC class 1 activity, like HD2, HDAC2, Histone deacetylase 2 HD2, HDAC2, Histone deacetylase 2
The HDAC Fluorescent Activity Assay kit is a complete assay system designed to measure histone deacetylase, HDAC, activity in cell extracts, nuclear extracts, immunoprecipitates, and purified enzymes. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent HDAC activity measurements. In addition, the kit includes purified HDAC2 and a potent HDAC inhibitor, TrichostatinA, for the positive and negative control. The HDAC Fluorescent Activity Assay is based on the unique fluorogenic substrate and Developer combination. This assay method eliminates dealing with the radioactivity, extraction, and chromatography aspects of traditional assays. Using this kit, only two simple steps on a microtiter plate are needed to analyze the HDAC activity level. First, the HDAC fluorometric substrate, comprised of an acetylated lysine side chain, is incubated with a sample containing HDAC activity, e.g. HeLa nuclear extract or purified HDAC enzymes. The deacetylation will sensitize the substrate, causing further treatment with the Lysine Developer to produce a fluorophore that can then be measured using a fluorescence reader.
Products for: HDAC1_2 - histone deacetylase 1/2 [EC:188.8.131.52]