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BPS-40690
VPS34, active human recombinant protein, N-​terminal FLAG-​tag
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20 µg

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VPS34, active human recombinant protein, N-terminal FLAG-tag


Enzyme kinetics, inhibitor screening, selectivity profiling Erklärungen der Abkürzungen ansehen.

Human VPS34 (vacuolar protein sorting 34), GenBank Accession No. NM_002647, a.a. 2-888(end) with N-terminal FLAG-tag, expressed in Sf9 insect cells via a baculovirus expression system. MW=98.2 kDa. Specific Activity: 140 pmole/min/µg. Enzyme reaction was conducted in a buffer (40 µl) containing 40 mM Tris (pH 7.4), 10 mM MgCl2, 2 mM MnCl2, 0.1 mg/ml BSA, 100 µM ATP, 100 µM PI:PS substrate (BPS # BPS-40560) and 0.6-5 ng/µl Vps34 at 30°C for 20 min. Kinase reaction was measured using ADP-Glo reagents (Promega). Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 µg/ml FLAG peptide, 20% glycerol. Stability: >6 months at -80°C. References: 1. Yan, Y., et al. (2009). Biochem J. 417(3):747-55. 2. Backer, J.M. (2008). Biochem J. 410(1):1-17. 3. Juhász, G., et al. (2008). J Cell Biol. 181(4):655-66.

MW: 98 kD
Purity: >49%

UniProt ID: Q8NEB9

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Products for: PIK3C3, VPS34 - phosphatidylinositol 3-kinase [EC:2.7.1.137]



Schlagworte:
PIK3C3, VPS34, hVps34, EC=2.7.1.137, PtdIns-​3-​kinase type 3, PI3K type 3, PI3-​kinase type 3, Phosphatidylinositol 3-​kinase catalytic subunit type 3, Phosphoinositide-​3-​kinase class 3, Phosphatidylinositol 3-​kinase p100 subunit VPS34, PIK3C3, hVps34, PI3K type 3, EC=2.7.1.137, PI3-​kinase type 3, PtdIns-​3-​kinase type 3, Phosphoinositide-​3-​kinase class 3, Phosphatidylinositol 3-​kinase p100 subunit, Phosphatidylinositol 3-​kinase catalytic subunit type 3

 

 

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