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IGF-1R, active human recombinant protein

ART NR.: BPS-40240 (10 µg)

Hersteller: BPS

Datenblatt

Anwendungen: Enzyme kinetics, inhibitor screening

MW: 72 kD

Reinheit: >80%

Format: solution

UniProt ID: P08069
Suche mit UniProt ID

Geninformation: NM_000875

Schlagworte: IGF1R, CD221, EC=2.7.10.1, IGF-I receptor, Insulin-like growth factor 1 receptor, Insulin-like growth factor I receptor, Insulin-like growth factor 1 receptor beta chain, Insulin-like growth factor 1 receptor alpha chain

BPS-40240

10 µg

335 €

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Bemerkungen: Active human IGFR1 (GenBank Accession No. NP_000875) MW=72.3 kDa. GST fusion protein containing the 46 kDa cytoplasmic domain of the beta-subunit of the human Insulin-like Growth Factor-1 Receptor Kinase, residues 930-1337, expressed in a baculovirus expression system. Source: Baculovirus infected Sf9 cells Formulated in: 1.0 mg/ml in 50 mM Tris, pH 7.5 and 1 mM DTT. Stability: >6 months at ?80°C Purity: >80% by SDS-PAGE. Specific activity: ~100 nmol/min/mg at 25°C using poly(Glu:Tyr)4:1 substrate. Reaction conditions are 50 mM Tris, pH 7.5, 5 mM MgCl2, 5 mM MnCl2, 1 mM DTT, 250 µM ATP. The kinase concentration was 0.5-1 µM and poly-L-lysine was added in equimolar amounts to the kinase. Applications: Enzyme kinetics, autophosphorylation, and phosphorylation of target substrates. References: 1. K. Baer, et al. Biochemistry 2001 40 14268 2. N. Tennagels, et al. Biochem. Biophys. Res. Com. 1999 260 7

Spezifikationen

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