EphB4, active human recombinant protein

Active human EPHB4 ( GenBank Accession No. NM_004444), (a.a. 563-987) with N-terminal His tag, MW=50 kDa, expressed in a Baculovirus infected Sf9 cell expression system. One unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to tyrosine substrate per minute at pH 7.4 and 30° C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM Na-orthovanadate, 0.1 mM ATP, 30 µg/ml Poly (Glu:Tyr)4:1 substrate, and 2 µg /ml recombinant EPHB4. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.