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ABD-12601
Amplite(TM) Fluorimetric beta-​Galactosidase Assay Kit *Green Fluorescence*
AAT Bioquest
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manual
1 kit

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195 €

Amplite(TM) Fluorimetric beta-Galactosidase Assay Kit *Green Fluorescence*


Galactosidase assays Erklärungen der Abkürzungen ansehen.

E. coli beta-galactosidase is a 464 kD tetramer. Each unit of beta-galactosidase consists of five domains, the third of which is the active site. It is an essential enzyme in cells. Deficiencies in this enzyme can result in galactosialidosis or Morquio B syndrome. In E. coli, beta-galactosidase is produced by the activation of LacZ operon. Detection of LacZ expression has become routine to the point of detection of as few as 5 copies of beta-galactosidase per cell. This kit uses a fluorogenic galactosidase substrate that can sensitively distinguish LacZ vs. LacZ-cells. It can be used either for detecting galactosidase conjugates in ELISA type assay systems or for monitoring LacZ gene expression in cells. The galactosidase-cleaved product has Ex/Em = 490/520 nm that can be detected with most of fluorescence instruments equipped with a FITC filter set.

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- Manual



Schlagworte:
EC=3.2.1.23

 

 

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