p70 S6 Kinase, Recombinant, Human (p70 S6K)

Applications: , Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested.. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Specific Activity: ~2pmol/min/ug , Assay Conditions: , Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35 and 2uM substrate (SER/THR 7) at RT for 1 hour. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months.