cdc42 Western Blot Kit, BioAssay,

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Applications: Suitable for use in detection of cdc42 by Western Blot. Quantity: 10 blots, Specificity: Recognizes cdc42 from human, mouse, rat and canine, Kit Components: Anti-cdc42, (mouse monoclonal IgG1):, One vial containing 50ug of purified mouse IgG1 in 200ul of 20mM sodium phosphate, pH 7.5, 150mM NaCl, 1.5mM sodium azide containing 50% glycerol and 1mg/ml BSA., This antibody was raised against a fusion protein corresponding to residues 1-191 of full length human cdc42. Liquid at-20°C. Blocking Reagent: One vial containing 20g non-fat skim milk fortified with vitamin A palmitate and vitamin D2, containing no preservatives. Goat Anti-Mouse IgG, HRP-Conjugate, (goat polyclonal IgG): One vial containing 500ug of immunoaffinity purified goat IgG conjugated to horseradish peroxidase lyophilized from 0.02M Potassium Phosphate, 0.15M NaCl, pH 7.2, 10mg/ml BSA, and 0.01% gentamicin sulfate. Storage and Stability: Stable for 1 year at-20°C from date of shipment. Note: Goat Anti-Mouse IgG (HRP) is light sensitive, handle accordingly.