UTX(KDM6A) Homogeneous Assay Kit

The UTX Homogeneous Assay Kit is designed to measure the activity of UTX for screening and profiling applications. UTX, also known as KDM6A, is a JmjC-domain protein that exhibits demethylation activity toward di- and trimethyl-lysine 27 (H3K27me2/3) on histone H3. The UTX Homogeneous Assay Kit comes in a convenient AlphaLISA(R) format, with biotinylated histone H3 peptide substrate, primary antibody, demethylase assay buffer, and purified UTX for 384 enzyme reactions. The key to the UTX Homogeneous Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for demethylase activity detection. First, UTX enzyme is incubated with the biotinylated substrate. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.