Tumor Necrosis Factor alpha (TNFa), porcine recombinant (rpTNFa)

Tumor Necrosis Factor alpha (TNFa), porcine recombinant (rpTNFa)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
T9160-15P1.5 5 µg - -

3 - 19 business days*

598.00€
T9160-15P1.20 20 µg - -

3 - 19 business days*

757.00€
 
TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their... more
Product information "Tumor Necrosis Factor alpha (TNFa), porcine recombinant (rpTNFa)"
TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK-cells following their stimulation by bacterial LPS . Cells expressing CD4 secrete TNF-alpha while CD8 cells secrete little or no TNF-alpha. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2 , GM-CSF. Description: Recombinant Porcine TNF-a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 157 amino acids and having a molecular mass of 17,274 Dalton. Porcine TNF-alpha is purified by standard chromatographic techniques. Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Arg-Ser-Ser. Dimers and Aggregates: Less than 1% as determined by silver-stained SDS-PAGE gel analysis. Biological Activity: Porcine TNF-alpha is fully biologically active when compared to standard. The ED50 as determined by the cytotoxicity of porcine PK15 cells was found to be < 0.008ng/ml. Endotoxin: < 0.1ng/ug (IEU/ug) of Porcine TNF-alpha. Protein Content: Protein quantitation was carried out by two independent methods:, 1. UV spectroscopy at 280 nm using the absorbency value of 1.26 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics)., 2. Analysis by RP-HPLC, using a calibrated solution of TNF-alpa as a Reference Standard. Solubility: It is recommended to reconstitute the lyophilized Porcine TNF-alpha in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. Stability: Lyophilized Porcine TNF-a although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution TNF-alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Keywords: swine
Supplier: United States Biological
Supplier-Nr: T9160-15P1

Properties

Conjugate: No
Host: E.coli
Species reactivity: swine
Format: Highly Purified

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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