Tumor Necrosis Factor alpha (TNFa), human recombinant (rHuTNFa)

Tumor Necrosis Factor alpha (TNFa), human recombinant (rHuTNFa)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
T9160-03H.100 100 µg - -

3 - 19 business days*

424.00€
T9160-03H.500 500 µg - -

3 - 19 business days*

742.00€
 
Tumor necrosis factor alpha (TNF-a), also called cachectin, consists of 158 amino acids. TNF-a is... more
Product information "Tumor Necrosis Factor alpha (TNFa), human recombinant (rHuTNFa)"
Tumor necrosis factor alpha (TNF-a), also called cachectin, consists of 158 amino acids. TNF-a is a 17.5kD factor produced by neutrophils, CD4+ T cells, macrophage, NK cells, LAK cells, astrocytes endothelial cells, and smooth muscle cells. TNF-a is cytolytic and plays an important role in immune regulation including hemorrhagic tumor necrosis/cytotoxicity and inflammation, and in regulation of antiviral and immune proliferative and activation responses. The active form of this protein is a trimer. Additional amino acid, methionine, was attached at N-terminus of the protein. Amino Acid Sequence: MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY , SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY , LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL, Biological Activity: Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is <0.16ng/ml. Activity Assay: L929 cytotoxicity assay using actinomycin D, Cell Number: 2 x 10,000 cells/well, Incubation: 24hr after sample treatment, Assay Media: 5% FBS media, Cytokine Concentration: 12 pg/ml - 25 ng/ml, Detection method: MTT assay, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Supplier: United States Biological
Supplier-Nr: T9160-03H

Properties

Conjugate: No
Host: E.coli
Species reactivity: human
Format: Highly Purified

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
You will get a certificate here
or to request a certificate of analysis.
Read, write and discuss reviews... more
Customer review for "Tumor Necrosis Factor alpha (TNFa), human recombinant (rHuTNFa)"
Write a review
or to review a product.
Viewed