Tumor Necrosis Factor alpha (TNFa), Highly Purified

Tumor Necrosis Factor alpha (TNF-alpha) is a 17.3kD multi-functional pro-inflammatory cytokine primarily secreted by macrophages, but also produced by T and B lymphocytes, activated monocytes and fibroblasts. TNF-alpha exists in both a transmembrane and mature soluble form and has many biological properties, including activation of transcription factor NF-KappaB, the induction of apoptosis in various tumor cell lines and the stimulation of cell differentiation and proliferation under certain conditions. Studies have revealed a role for TNF-alpha in inflammation, septic shock, autoimmune disease and rheumatoid arthritis. Biological Properties: Recombinant rat TNF alpha was tested in a cytotoxicity assay on murine L929 cells-this batch demonstrated an ED50 of < 0.5ng/ml. Applications: Suitable for use in ELISA, Western Blot, and Funtional Assays. Other applications have not been tested. Recommended Dilutions: ELISA: 0.2-0.4ng/well , Western Blot: 1.5-3.0ng/lane Optimal working dilutions to be determined by researcher. Storage and Stability: May be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.