TMB Stable Liquid Substrate 1.25mM/Liter for HRPO (3,3', 5,5'-Tetramethylbenzidine) (3,3',5,5'-Tetra

Item number	Size	Manual	SDS	Delivery time	Price
T6005.100	100 ml	-	-	3 - 19 business days*	228.00€
T6005.250	250 ml	-	-	3 - 19 business days*	363.00€
T6005.1000	1000 ml	-	-	3 - 19 business days*	534.00€

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ImmunoTek(TM) TMB (3,3',5,5'-Tetramethylbenzidine) Stable Liquid Substrate for HRPO, 1.25mM,... more

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Product information "TMB Stable Liquid Substrate 1.25mM/Liter for HRPO (3,3', 5,5'-Tetramethylbenzidine) (3,3',5,5'-Tetra"

ImmunoTek(TM) TMB (3,3',5,5'-Tetramethylbenzidine) Stable Liquid Substrate for HRPO, 1.25mM, Soluble, Tetramethylbenzidine (TMB) is an excellent substrate for detecting horseradish peroxidase (HRP) labeled probes. TMB produces a soluble end product which is blue in color that is quantitated at 650nm, providing an ultrasensitive quantitative substrate system. TMB has been shown to be a safe-sensitive substrate for the assay of horseradish peroxidase (HRP). Initially, in the presence of HRP and hydrogen peroxide, a one-electron oxidation product is formed. This compound, a cation free radical, is blue in color wth an adsorption maximum at 653nm. Further reaction with HRP/H2O2 or acidification of the radical with acid yields the diimine terminal oxidation product adsorbing light at 450 nm. The extinction coefficient of the radical (E653 nm = 5.9x10e4 mol-1 cm-1) provide a remarkably sensitive system for the assay of HRP and HRP labeled probes. TMB is a single component reagent stable at room temperature and not sensitive to normal laboratory light. It is optimized with respect to TMB and hydrogen peroxide concentrations and yields a linear response with the concentrations of HRP usually employed in immunologic assays.

Supplier:	United States Biological
Supplier-Nr:	T6005

Properties

Application:	ELISA
Format:	Molecular Biology Grade

Database Information

CAS :	4827-17-7\| Matching products
UniProt ID :	P00433 \| Matching products

Handling & Safety

Storage:	+4°C
Shipping:	+20°C (International: +20°C)

Caution
Our products are for laboratory research use only: Not for administration to humans!

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