TAF1 (BD1/BD2) Inhibitor Screening Assay Kit

The TAF1 (BD 1 BD2) Inhibitor Screening Assay Kit is designed to measure the inhibition of TAF1 binding to its substrate. The kit comes in a convenient AlphaLISA format, with enough biotinylated histone peptide substrate, assay buffer, detection buffer and purified GST-tagged TAF1 (bromodomains 1 and 2) to perform a total of 384 enzyme reactions. The key to the kit is the specific binding of the tandem TAF1 bromodomains to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing TAF1 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts.