Smad2, Human (Small Mothers against Decapentaplegic Deleted in Pancreatic Carcinoma), BioAssay ELISA

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmits TGF-b signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5 and 8, the common-mediator Smad (co-Smad), Smad4, and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8). Kit Components: Smad2 mAb coated microtiter plate, 1x96 tests , Smad2 Detection Ab, 1x11ml , Anti-mouse IgG, HRP-linked Antibody, 1x11 ml, TMB Substrate, 1x11ml, STOP Solution, 1x11ml, ELISA Wash Buffer (20X), 1x25ml , ELISA Sample Diluent, 1x25ml, Cell Lysis Buffer (10X), 1x 15ml, Storage and Stability: Store components at 4°C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.