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Screen Quest(TM) Fura-​2 No Wash Calcium Assay Kit Intracellular Calcium homog. Fluorometr. Sensors Cellular assays Calcium flux assaysErklärungen der Abkürzungen ansehen. AAT Bioquest
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ABD-​36320 10 plates

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395 €In Warenkorb aufnehmen

Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This ratiometric calcium assay kit allows homogeneous measurement of intracellular calcium changes caused by activation of G-​protein-​coupled receptors or calcium channels. Cells expressing a GPCR of interest that signals through calcium are pre-​loaded with Fura-​2 AM which can cross cell membrane. Once inside the cell, the lipophilic blocking groups of Fura-​2 AM are cleaved by esterases, resulting in a negatively charged fluorescent dye that stays inside cells and its fluorescence wavelength is blue-​shifted upon binding to calcium. When cells stimulated with agonists, the receptor signals the release of intracellular calcium, which greatly increase the fluorescence intensity ofFura-​2 at the short wavelength. The ratiometric characteristics of Fura-​2 make this kit an ideal tool for more accurate measurement of cellular calcium concentration compared to Fluo-​4 of the single wavelength. With a single addition, the assay is easy to perform and desirable in a high thoroughput environment. The assay can be used in a convenient 96-​well or 384-​well microtiter-​plate format and readily adapted to automation.

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Kat #:
ABD-36320
Menge:
10 plates

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Preis:
395 €

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