Screen Quest(TM) Fluorimetric Glucose Uptake Assay Kit

Screen Quest(TM) Fluorimetric Glucose Uptake Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-36500 100 tests -

3 - 8 business days*

978.00€
ABD-36501 500 tests -

3 - 8 business days*

4,143.00€
 
Ex: 571 nm. Em: 585 nm. Glucose transport systems are responsible for transporting glucose across... more
Product information "Screen Quest(TM) Fluorimetric Glucose Uptake Assay Kit"
Ex: 571 nm. Em: 585 nm. Glucose transport systems are responsible for transporting glucose across cell membranes. Measuring uptake of 2-deoxyglucose (2-DG), a glucose analog, in tissues and cells is widely accepted as a reliable method to estimate the amount of glucose uptake and to investigate the regulation of glucose metabolism and mechanism of insulin resistance. The 2-DG uptake is commonly determined by using non-metabolized 2-DG labeled with tritium or C14. However, routine use of a radiolabelled probe is costly and requires a tedious special handling procedure. AAT Bioquest's Screen QuestT Fluorimetric Glucose Uptake Assay Kit provides a sensitive and non-radioactive assay in tissues or cultured cells. In this assay 2-DG is taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). The non-metabolizable 2-DG6P accumulates in the cells, and is proportional to glucose uptake by cells. The accumulated 2-DG6P is enzymatically oxidized and generates NADPH, which is specifically monitored by a fluorogenic NADPH sensor. The signal can be read by a fluorescence microplate reader at Ex/Em = 540 nm/590 nm.
Supplier: AAT Bioquest
Supplier-Nr: 36500

Properties

Application: HTS, Glucose uptake assay
Format: Homog.

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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