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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
ABD-36500 | 100 tests | - |
3 - 8 business days* |
978.00€
|
|||
ABD-36501 | 500 tests | - |
3 - 8 business days* |
4,143.00€
|
Ex: 571 nm. Em: 585 nm. Glucose transport systems are responsible for transporting glucose across... more
Product information "Screen Quest(TM) Fluorimetric Glucose Uptake Assay Kit"
Ex: 571 nm. Em: 585 nm. Glucose transport systems are responsible for transporting glucose across cell membranes. Measuring uptake of 2-deoxyglucose (2-DG), a glucose analog, in tissues and cells is widely accepted as a reliable method to estimate the amount of glucose uptake and to investigate the regulation of glucose metabolism and mechanism of insulin resistance. The 2-DG uptake is commonly determined by using non-metabolized 2-DG labeled with tritium or C14. However, routine use of a radiolabelled probe is costly and requires a tedious special handling procedure. AAT Bioquest's Screen QuestT Fluorimetric Glucose Uptake Assay Kit provides a sensitive and non-radioactive assay in tissues or cultured cells. In this assay 2-DG is taken up by glucose transporters, and metabolized to 2-DG-6-phosphate (2-DG6P). The non-metabolizable 2-DG6P accumulates in the cells, and is proportional to glucose uptake by cells. The accumulated 2-DG6P is enzymatically oxidized and generates NADPH, which is specifically monitored by a fluorogenic NADPH sensor. The signal can be read by a fluorescence microplate reader at Ex/Em = 540 nm/590 nm.
Supplier: | AAT Bioquest |
Supplier-Nr: | 36500 |
Properties
Application: | HTS, Glucose uptake assay |
Format: | Homog. |
Database Information
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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