Screen Quest(TM) Fluorimetric ELISA cAMP Assay Kit

Screen Quest(TM) Fluorimetric ELISA cAMP Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-36373 1 plate -

3 - 8 business days*

418.00€
ABD-36374 10 plates -

3 - 8 business days*

2,741.00€
 
Screen Quest(TM) cAMP Fluorescence ELISA Assay Kit provides an optimized assay method for... more
Product information "Screen Quest(TM) Fluorimetric ELISA cAMP Assay Kit"
Screen Quest(TM) cAMP Fluorescence ELISA Assay Kit provides an optimized assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems. The assay is based on the competition for a fixed number of antibody binding sites between HRP-labeled cAMP and non-labeled cAMP. HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex by unlabeled free cAMP. In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. However, the unlabeled free cAMP in the test sample competes for anti-cAMP antibody with the HRP-cAMP antibody conjugate, therefore inhibits the binding of HRP-cAMP to anti-cAMP antibody. Our Screen Quest(TM) cAMP Fluorometric Assay Kit provides a sensitive method for detecting adenylate cyclase activity. Compared to other commercial ELISA cAMP assay kits, this cAMP assay kit only requires a single wash step to remove unbound material prior to the development step. It also eliminates the tedious acetylation step. The kit uses Amplite(TM) Red as a fluorogenic HRP substrate to quantify the HRP activity. The fluorescent product formed is proportional to the activity of HRP-cAMP conjugate.
Keywords: cyclic AMP
Supplier: AAT Bioquest
Supplier-Nr: 36373

Properties

Application: cAMP ELISA
Format: Solid Phase

Database Information

Handling & Safety

Storage: +4°C
Shipping: +4°C (International: +4°C)
Signal Word: Warning
Caution
Our products are for laboratory research use only: Not for administration to humans!
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