Screen Quest(TM) Colorimetric ELISA cAMP Assay Kit

Screen Quest(TM) Colorimetric ELISA cAMP Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
ABD-36370 1 plate -

3 - 8 business days*

418.00€
ABD-36371 10 plates -

3 - 8 business days*

2,741.00€
 
Adenosine 3', 5' cyclic monophosphate (cAMP) is an important second messenger in intracellular... more
Product information "Screen Quest(TM) Colorimetric ELISA cAMP Assay Kit"
Adenosine 3', 5' cyclic monophosphate (cAMP) is an important second messenger in intracellular signal transduction. Monitoring levels of cAMP is one of the most common ways to screen for agonists and antagonists of GPCRs. Screen Quest(TM) cAMP Colorimetric ELISA Assay Kit is based on the competition between HRP-labeled cAMP and non-labeled cAMP. HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex by unlabeled free cAMP. In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. However, the unlabeled free cAMP in the test sample competes for anti-cAMP antibody with the HRP-cAMP antibody conjugate, therefore inhibits the binding of HRP-cAMP to anti-cAMP antibody. Our Screen Quest(TM) cAMP Colorimetric ELISA Assay Kit provides a sensitive method for detecting adenylate cyclase activity in biochemical or cell-based assay system. Compared to other ELISA cAMP assay kits, our kit eliminates the tedious acetylation step. The kit uses Amplite(TM) Blue as a colorimetric substrate to quantify the HRP activity. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
Keywords: cyclic AMP
Supplier: AAT Bioquest
Supplier-Nr: 36370

Properties

Application: cAMP ELISA
Format: Solid Phase

Database Information

Handling & Safety

Storage: -20°C
Shipping: +4°C (International: +4°C)
Signal Word: Warning
Caution
Our products are for laboratory research use only: Not for administration to humans!
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