SIRT3 Direct Fluorescent Screening Assay Kit

SIRT3 Direct Fluorescent Screening Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay10011566-96 96 wells -

6 - 10 business days*

467.00€
 
The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class... more
Product information "SIRT3 Direct Fluorescent Screening Assay Kit"
The sirtuins represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs) that catalyze a reaction coupling lysine deacetylation to the formation of nicotinamide and O-acetyl-ADP-ribose. SIRT3 is a mitochondrial protein that plays a role in metabolic regulation and other cellular processes. It is the only human sirtuin with a direct genetic link with longevity. Additional research needs to be done to further elucidate the mechanism behind the involvement of SIRT3 in metabolic dysfunction, aging, cancer, and neurodegenerative disease. Cayman's SIRT3 Direct Fluorescent Screening Assay Kit provides a convenient fluorescence-based method for screening SIRT3 inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which comprises the p53 sequence Gln-Pro-Lys-Lys(e-acetyl)-AMC, is incubated with human recombinant SIRT3 along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product. The fluorophore can be analyzed with an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm.License: Sold under license from Cyclex..
Keywords: SIRT3, hSIRT3, SIR2L3, EC=3.5.1.-, SIR2-like protein 3, NAD-dependent deacetylase sirtuin-3, mitochondrial
Supplier: Cayman Chemical
Supplier-Nr: 10011566

Properties

Application: SIRT3 activator/inhibitor screening
Species reactivity: human
Format: Homog.

Database Information

KEGG ID : K11413 | Matching products
UniProt ID : Q9NTG7 | Matching products

Handling & Safety

Storage: -80°C
Shipping: -80°C (International: -80°C)
Signal Word: Warning
GHS Hazard Pictograms:
H Phrases: H316, H319
P Phrases: P264, P280, P305+351+338, P332+313, P337+313
Caution
Our products are for laboratory research use only: Not for administration to humans!
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