SIRT1 FRET-Based Screening Assay Kit

SIRT1 FRET-Based Screening Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay10010991-96 96 wells -

6 - 10 business days*

467.00€
 
Sirtuins (SIRTs) represent a distinct class of trichostatin A-insensitive lysyl-deacetylases... more
Product information "SIRT1 FRET-Based Screening Assay Kit"
Sirtuins (SIRTs) represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs). Human SIRT1 is the homolog of yeast Sir2 (Silent information regulator 2) and has been shown to regulate the activity of the p53 tumor suppressor and inhibit apoptosis. Small molecule activators of SIRT1, such as resveratrol, extend lifespan in yeast and C. elegans in a manner that resembles caloric restriction. Cayman's SIRT1 FRET-based Screening Assay Kit provides a convenient fluorescence-based method for screening SIRT1 inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which is coupled to the fluorophore and quencher, is incubated with human recombinant SIRT1 along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step results in the separation of the quencher and fluorophore. The resulting fluorescence can be easily analyzed using an excitation wavelength of 335-345 nm and emission wavelength of 440-465 nm.License: Sold under license from Cyclex..
Keywords: SIRT1, hSIR2, hSIRT1, SIR2L1, EC=3.5.1.-, SIR2-like protein 1, NAD-dependent deacetylase sirtuin-1
Supplier: Cayman Chemical
Supplier-Nr: 10010991

Properties

Application: SIRT1 inhibitor/activator screening
Species reactivity: human
Format: Homog.

Database Information

KEGG ID : K11411 | Matching products
UniProt ID : Q96EB6 | Matching products

Handling & Safety

Storage: -80°C
Shipping: -80°C (International: -80°C)
Signal Word: Danger
GHS Hazard Pictograms:
H Phrases: H303, H316, H319, H334, H335
P Phrases: P261, P264, P271, P280, P284, P304+340, P304+341, P305+351+338, P312, P332+313, P337+313, P342+311, P403+233, P405, P501
Caution
Our products are for laboratory research use only: Not for administration to humans!
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