SIRT1 Direct Fluorescent Screening Assay Kit

SIRT1 Direct Fluorescent Screening Assay Kit
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay10010401-96 96 wells -

6 - 10 business days*

467.00€
 
Sirtuins (SIRTs) represent a distinct class of trichostatin A-insensitive lysyl-deacetylases... more
Product information "SIRT1 Direct Fluorescent Screening Assay Kit"
Sirtuins (SIRTs) represent a distinct class of trichostatin A-insensitive lysyl-deacetylases (class III HDACs). Human SIRT1 is the homolog of yeast Silent information regulator 2 (Sir2) and has been shown to regulate the activity of the p53 tumor suppressor and inhibit apoptosis. Small molecule activators of SIRT1, such as resveratrol, extend lifespan in yeast and C. elegans in a manner that resembles caloric restriction. However, this activation of SIRT1 is under much debate. It was determined that the in vitro activation of SIRT1 and Sir2 by resveratrol was a substrate-specific event (i.e., the binding of resveratrol to SIRT1 promoted a conformational change that better accommodated the attached coumarin group in the peptide substrate). Additional research needs to be done to further elucidate the mechanism behind SIRT1 activation, as well as, to establish its role in aging, cancer, and neurodegenerative disease. Cayman's SIRT1 Direct Fluorescent Screening Assay Kit provides a convenient fluorescence-based method for screening SIRT1 inhibitors or activators. The procedure requires only two easy steps, both performed in the same microplate. In the first step, the substrate, which comprises the p53 sequence Arg-His-Lys-Lys(e-acetyl)-AMC, is incubated with human recombinant SIRT1 along with its cosubstrate NAD+. Deacetylation sensitizes the substrate such that treatment with the developer in the second step releases a fluorescent product. The fluorophore can be easily analyzed using an excitation wavelength of 350-360 nm and an emission wavelength of 450-465 nm.License: Sold under license from Cyclex..
Keywords: SIRT1, hSIR2, hSIRT1, SIR2L1, EC=3.5.1.-, SIR2-like protein 1, NAD-dependent deacetylase sirtuin-1
Supplier: Cayman Chemical
Supplier-Nr: 10010401

Properties

Application: SIRT1 inhibitor/activator screening
Species reactivity: human
Format: Homog.

Database Information

KEGG ID : K11411 | Matching products
UniProt ID : Q96EB6 | Matching products

Handling & Safety

Storage: -80°C
Shipping: -80°C (International: -80°C)
Signal Word: Danger
GHS Hazard Pictograms:
H Phrases: H227, H315, H316, H319, H334, H335
P Phrases: P210, P261, P264, P271, P280, P284, P302+352, P304+340, P304+341, P305+351+338, P312, P321, P332+313, P337+313, P342+311, P362+364, P370+378, P403, P403+233, P405, P501
Caution
Our products are for laboratory research use only: Not for administration to humans!
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