SET7/9 SAM-Screener(TM) Assay Kit (384 wells)
ART NR.: Cay600490-384 (384 wells)
Cay600490-1920 (1920 wells)
Anwendungen: Inhibitor screening, HTS
Messtechnik: Fluor. Polar.
UniProt ID: Q8WTS6
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Schlagworte: SETD7, SET7/9, KIAA1717, EC=188.8.131.52, H3-K4-HMTase SETD7, Lysine N-methyltransferase 7, SET domain-containing protein 7, Histone H3-K4 methyltransferase SETD7, Histone-lysine N-methyltransferase SETD7
Most histone lysine methyltransferases contain a conserved domain (SET) that utilizes S-adenosyl-L-methionine (SAM or AdoMet) as a co-factor to catalyze the methylation of lysine. SET7/9 (KMT7) is a SET domain-containing mono-methyltransferase that acts on a large number of histone and non-histone targets including histone H3, TAF10, p53, viral Tat, and estrogen receptor a. Given this broad substrate specificity, SET7/9 has important implications for a variety of human diseases, and SET7/9 has emerged as a model for studying the catalytic mechanism SET domain histone methyltransferases. This fluorescence polarization assay is based upon a proprietary small molecule fluorescent probe that binds to the SAM binding pocket in SET7/9. Binding of the small molecule probe to SET7/9 induces an increase in fluorescence polarization. Binding of the probe can be competed with the endogenous cofactor SAM or by the inhibitor sinefungin, but is unaffected by the histone H3 peptide substrate. The SET7/9 SAM-Binding Site Inhibitor Screening Assay is robust (Z' >0.6) and exhibits a greater than 100 mP shift over a range of 0-250 nM SET7/9. The assay is suitable for high-throughput screening in the provided 384-well plate or can be scaled to higher density plate formats (e.g., 1536-well) if desired.
Products for: SETD7 - histone-lysine N-methyltransferase SETD7 [EC:184.108.40.206]
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