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SET7/9 Methyltransferase Inhibitor Screening Assay Kit
Gene Regul. human
Erklärungen der Abkürzungen ansehen.
homog. Fluorometr. Enzymatic Purified Enzymes Inhibitor screeningErklärungen der Abkürzungen ansehen. Cayman
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Cay700270-​96 96 wells

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Cayman's SET7/9 Methyltransferase Inhibitor Screening Assay provides a convenient method for screening human SET7/9 inhibitors. The transfer of the methyl group from SAM by SET7/9 to the acceptor peptide (TAF 10) generates S-​adenosylhomocysteine, which is rapidly converted to S-​ribosylhomocysteine and adenine by adenosylhomocysteine nucleosidase. This rapid conversion prevents the buildup of adenosylhomocysteine and its feedback inhibition on the methylation reaction. Finally, the adenine is converted to hypoxanthine by adenine deaminase, which in turn is converted to urate and hydrogen peroxide (H2O2). The reaction between H2O2 and ADHP (10-​acetyl-​3,7-​dihydroxyphenoxazine) produces the highly fluorescent compound resorufin. Resorufin fluorescence is analyzed using an excitation wavelength of 530-​540 nm and an emission wavelength of 585-​595 nm.

Datenblatt ansehen
Kat #:
Cay700270-96
Menge:
96 wells

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Preis:
585 €

UniProt ID: Q8WTS6 (Protein knowledgebase)

Schlagworte:
SETD7, SET7/9, KIAA1717, EC=2.1.1.43, H3-​K4-​HMTase SETD7, Lysine N-​methyltransferase 7, SET domain-​containing protein 7, Histone H3-​K4 methyltransferase SETD7, Histone-​lysine N-​methyltransferase SETD7
Image Art 700270 Cayman SET7/9 Methyltransferase Inhibitor Screening Assay Kit

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Products for: SETD7 - histone-lysine N-methyltransferase SETD7 [EC:2.1.1.43]



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