Resistin (Adipose Tissue-Specific Secretory Factor, ADSF, Found in Inflammatory Zone, FIZZ3, Adipose

Resistin, a product of the RSTN gene, is a peptide hormone belonging to the class of cysteine-rich secreted proteins (monomeric peptide contains 11 cysteine residues) referred to as the RELM family, and is also described as ADSF (Adipose Tissue-Specific Secretory Factor) or FIZZ3 (Found in Inflammatory Zone 3). Mouse resistin is expressed as a 114 amino acid prepeptide, its hydrofobic Nterminal 20 amino acid signal peptide is cleaved before its secretion. Mouse resistin circulates in blood as a homodimeric protein consisting of two 94 amino acid polypeptides, which are disulfide-linked via Cys26. Resistin may be an important link between obesity and insulin resistance. Mouse resistin, specifically produced and secreted by adipocyte, acts on skeletal muscle myocytes, hepatocytes and adipocytes themselves so that it reduces their sensitivity to insulin. Steppan et al. have suggested that resistin suppressed the ability of insulin to stimulate glucose uptake. They have also suggested that resistin was present at elevated levels in blood of obese mice, and was down regulated by fasting and by antidiabetic drugs. Way et al., on the other hand, have found that resistin expression is severely suppressed in obesity and is stimulated by several antidiabetic drugs. Other studies have shown that mouse resistin increases during the differentiation of adipocytes, but it also seems to inhibit adipogenesis. In contrast, the human adipogenic differentiation is likely to be associated with a down regulation of resistin gene expression. , Source:, A DNA sequence encoding the mature mouse Resistin protein sequence Ser 21-Ser 114 (Steppan, C.M. et al., 2001, Nature 409:307-312) was expressed in E. coli. Molecular Mass: Recombinant mouse Resistin preparation contains a mixture of monomeric and disulfide-linked homodimeric proteins. Each monomer contains 94 amino acid residues and has a calculated molecular mass of approximately 10kD. Activity: Measured by its ability to inhibit mouse 3T3-L1 cell adipocyte differentiation (Kim, K.H. et al., 2001, J. Biol. Chem. 276:11252-11256). The ED50 for this effect is typically 5-25ug/ml. Endotoxin: < 0.1ng/ug (1EU/ug). , Reconstitution:, It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 10ug/ml. Storage and Stability: Lyophilized samples are stable for up to 12 months from date of receipt at -20°C to -70°C. Upon reconstitution, this cytokine can be stored under sterile conditions at 2-8° C for one month or at -20 to -70° C in a manual defrost freezer for three months without detectable loss of activity. Avoid repeated freeze-thaw cycles.