Renin Fluorogenic Substrate

The renin fluorogenic substrate consists of the normal peptide substrate for renin which has been linked to the fluorophore EDANS at one end and to a non-fluorescent quenching molecule (Dabcyl) at the other. After cleavage by renin, the product (peptide-EDANS) is brightly fluorescent and can be easily analyzed using an excitation wavelength of 340 nm and emission wavelengths of 485-510 nm.Formal Name: L-arginyl-N-[2-[(5-sulfo-1-naphthalenyl)amino]ethyl]-L-glutaminyl-L-isoleucyl-L-histidyl-L-prolyl-L-phenylalanyl-L-histidyl-L-leucyl-L-valyl-L-isoleucyl-L-histidyl-L-threonyl-N6-[4-[[4-(dimethylamino)phenyl]azo]benzoyl]-L-lysyl-L-arginine. CAS Number: 791068-69-4. Molecular Formula: C109H156N32O21S. Formula Weight: 2282.7. Purity: >95%. Emission: 490 nm. Excitation: 340 nm. Formulation: (Request formulation change), 1 mg of a crystalline solid. SMILES: O=C(CC[C@H](NC([C@@H](N)CCCNC(N)=N)=O)C(N[C@@H]([C@@H](C)CC)C(N[C@@H](CC1=CN=CN1)C(N(CCC2)[C@@H]2C(N[C@H](C(N[C@H](C(N[C@@H](CC(C)C)C(N[C@@H](C(C)C)C(N[C@@H]([C@@H](C)CC)C(N[C@H](C(N[C@@H]([C@H](O)C)C(N[C@@H](CCCCNC(C(C=C3)=CC=C3N=NC(C=C4)=CC=C4N(C)C)=O)C(N[C@H](C(O)=O)CCCNC(N)=N)=O)=O)=O)CC5=CN=CN5)=O)=O)=O)=O)CC6=CN=CN6)=O)CC7=CC=CC=C7)=O)=O)=O)=O)NCCNC8=CC=CC9=C8C=CC=C9[S](=O)(O)=O. InChi Code: InChI=1S/C109H156N32O21S/c1-12-63(7)90(135-96(147)79(126-94(145)76(110)28-21-45-121-108(111)112)42-43-88(143)119-48-47-118-77-30-19-27-75-74(77)26-20-33-87(75)163(160,161)162)104(155)133-85(54-72-57-117-60-125-72)106(157)141-49-23-32-86(141)101(152)131-82(51-66-24-15-14-16-25-66)97(148)130-83(52-70-55-115-58-123-70)98(149)129-81(50-61(3)4)99(150)134-89(62(5)6)102(153)136-91(64(8)13-2)103(154)132-84(53-71-56-116-59-124-71)100(151)137-92(65(9)142)105(156)127-78(95(146)128-80(107(158)159)31-22-46-122-109(113)114)29-17-18-44-120-93(144)67-34-36-68(37-35-67)138-139-69-38-40-73(41-39-69)140(10)11/h14-16,19-20,24-27,30,33-41,55-65,76,78-86,89-92,118,142H,12-13,17-18,21-23,28-29,31-32,42-54,110H2,1-11H3,(H,115,123)(H,116,124)(H,117,125)(H,119,143)(H,120,144)(H,126,145)(H,127,156)(H,128,146)(H,129,149)(H,130,148)(H,131,152)(H,132,154)(H,133,155)(H,134,150)(H,135,147)(H,136,153)(H,137,151)(H,158,159)(H4,111,112,121)(H4,113,114,122)(H,160,161,162)/t63-,64-,65+,76-,78-,79-,80-,81-,82-,83-,84-,85-,86-,89-,90-,91-,92-/m0/s1. UniProt Accession #: P00797.