Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This RPE-iFluor(TM) 647-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with RPE-iFluor(TM) 647 covalently attached (as the fluorescent label). It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a very valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a Cy5® filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.
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