Resazurin, which is the N-oxide of the fluorescent dye resorufin, is useful for detecting reductive activities in cells and has been widely used for measuring cell proliferation and mitochondrial metabolic activity. Resazurin itself is non-fluorescent until it is reduced to the highly red fluorescent resorufin...

The liver X receptors, LXRalpha and LXRbeta, are nuclear receptors that act as ligand-dependent transcription factors. They modulate cholesterol, fatty acids, and glucose homeostasis. GW 3965 is an orally-active agonist of LXRalpha and LXRbeta, activating the human isoforms with EC50 values of 190 and 30 nM,...

Glutamate is a key molecule in cellular metabolism. It is the most abundant excitatory neurotransmitter in the vertebrate nervous system. Nerve impulses trigger release of glutamate from the pre-synaptic cells. In the opposing post-synaptic cell, glutamate receptors, such as the NMDA receptor, bind glutamate and are...

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....