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Product information "Purification Media, Affinity, Protein G Sepharose"
Protein G originated from Streptococci is a monomeric protein with a molecular weight of about 50,000kD. Protein G binds with high affinity to the Fc region of most classes and subclasses of immunoglobulins from mouse, rabbit, goat and human. Due to this binding ability, Protein G is commonly used in the purification and recovery of either polyclonal or monoclonal immunoglobulins. Recombinant Protein G used in Protein G beads is a genetically engineered form of Protein G. Non-essential regions have been removed while leaving the IgG binding sites intact. The recombinant Protein G has been immobilized on Sepharose beads in order to create a versatile product for both the purification of various mammalian immunoglobulins and for isolating immune complexes by immunoprecipitation (IP). Matrix: SepharoseTM CL-4B, Coupling Method: TargetLock , Protein G Density: 3-4mg/ml of gel, Binding Capacity: ~4-8mg rabbit IgG per ml of beads, Mean Bead Size: 40-165um, Bead Structure: Highly cross-linked spherical agarose, 4%, Max Back Pressure: 0.3MPa, 3 bar, Max. Flow Rates: 4ml/min/cm2 , Recommended Flow Rate: 1-3 ml/min/cm2, Stability of the Matrix: pH 2-11. Storage: Store gel refrigerated at 4°C in PBS pH 7.4 containing 0.1% sodium azide as a preservative, or PBS pH 7.4 containing 20% ethanol.
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the usability of this website, serve for direct advertising or simplify interaction with other websites and social networks, will only be used with your consent.
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