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Prostaglandin D Synthase (hematopoietic-type) FP-Based Inhibitor Screening Assay Kit - Green

Bioactive Lipids

human

homog.

Fluor. Polar.

Sensors

Purified Enzymes

PGDS inhibitor screening

Cayman

Cay600007-384

384 wells

Buy Bulk

413 €

Cayman's H-PGDS FP-Based Inhibitor Screening Assay Kit - Green provides a rapid, accurate assay for screening H-PGDS inhibitors. In this assay, a H-PGDS inhibitor-fluorescein conjugate serves as a specific fluorescent probe for the enzyme. Displacement of the probe by any unlabeled H-PGDS inhibitor leads to a decrease in the fluorescence polarization (FP) state of the probe, providing a direct signal for binding of the inhibitor to the active site of the enzyme. This assay is robust (Z of 0.75) and eliminates the need for the traditional multistep assay that requires the use of highly unstable PGH2. The assay has been validated using several known inhibitors of H-PGDS with IC50 values ranging from nanomolar to millimolar concentrations.

Datenblatt ansehen

Kat #:

Cay600007-384

Menge:

384 wells

Buy Bulk

Preis:

413 €

UniProt ID: O60760 (Protein knowledgebase)

Schlagworte:
GSTS, HPGDS, H-PGDS, EC=2.5.1.18, EC=5.3.99.2, GST class-sigma, Glutathione S-transferase, Prostaglandin-H2 D-isomerase, Glutathione-dependent PGD synthase, Hematopoietic prostaglandin D synthase, Glutathione-requiring prostaglandin D synthase
Image Art 600007 Cayman Prostaglandin D Synthase (hematopoietic-type) FP-Based Inhibitor Screening Assay Kit - Green