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Prostaglandin D Synthase (hematopoietic-​type) FP-​Based Inhibitor Screening Assay Kit -​ Green
Bioactive Lipids human
Erklärungen der Abkürzungen ansehen.
homog. Fluor. Polar. Sensors Purified Enzymes PGDS inhibitor screeningErklärungen der Abkürzungen ansehen. Cayman
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Cay600007-​384 384 wells

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413 €In Warenkorb aufnehmen

Cayman's H-​PGDS FP-​Based Inhibitor Screening Assay Kit -​ Green provides a rapid, accurate assay for screening H-​PGDS inhibitors. In this assay, a H-​PGDS inhibitor-​fluorescein conjugate serves as a specific fluorescent probe for the enzyme. Displacement of the probe by any unlabeled H-​PGDS inhibitor leads to a decrease in the fluorescence polarization (FP) state of the probe, providing a direct signal for binding of the inhibitor to the active site of the enzyme. This assay is robust (Z of 0.75) and eliminates the need for the traditional multistep assay that requires the use of highly unstable PGH2. The assay has been validated using several known inhibitors of H-​PGDS with IC50 values ranging from nanomolar to millimolar concentrations.

Datenblatt ansehen
Kat #:
Cay600007-384
Menge:
384 wells

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Preis:
413 €

UniProt ID: O60760 (Protein knowledgebase)

Schlagworte:
GSTS, HPGDS, H-​PGDS, EC=2.5.1.18, EC=5.3.99.2, GST class-​sigma, Glutathione S-​transferase, Prostaglandin-​H2 D-​isomerase, Glutathione-​dependent PGD synthase, Hematopoietic prostaglandin D synthase, Glutathione-​requiring prostaglandin D synthase
Image Art 600007 Cayman Prostaglandin D Synthase (hematopoietic-​type) FP-​Based Inhibitor Screening Assay Kit -​ Green

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Products for: E5.3.99.2, PTGDS - prostaglandin-H2 D-isomerase [EC:5.3.99.2]




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