A Rat isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific?Rat antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for...

A Rat isotype control is an antibody of the same class (isotype) of immunoglobulin as the specific Rat antibody, but is either myeloma-derived antibody of unknown specificity, or is raised against an antigen that is presumed not to be present on or in the cells under study. Isotype control antibodies are used for...

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

Small GTPases are a super-family of cellular signaling regulators. Cdc42 belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Cdc42, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase...