Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides, derived from PUFAs, are unstable and decompose to form a complex series of compounds, which include reactive carbonyl compounds, such...

Cayman's beta-HB (Ketone Body) Fluorometric Assay Kit provides a simple, reproducible, and sensitive tool for assaying beta-HB from plasma, serum, urine, tissue homogenates, and cell lysates. The method for beta-HB determination is based upon the oxidation of D-3-Hydroxybutyrate to acetoacetate by the enzyme...

Glucose-6-phosphate (G6P) plays a key role in the homeostatic regulation of blood glucose levels by participating in glycolysis, glycogen synthesis, gluconeogenesis, and can be metabolized to NADPH, enabling protection against oxidative damage. Disruption of G6P activity leads to glycogen storage disease type I or...

Cayman's Glycogen Assay provides a simple, reproducible, and sensitive tool for measuring glycogen in tissue/cells. Glycogen is hydrolyzed by amyloglucosidase to form beta-D-glucose, which is then specifically oxidized by glucose oxidase to form hydrogen peroxide. Hydrogen peroxide, in the presence of horseradish...