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PPARgamma Transcription Factor Assay Kit

ART NR.: Cay10006855-96 (96 wells)

Hersteller: Cayman

Datenblatt



MSDS

Anwendungen: PPARgamma binding detection

Format: solid phase

UniProt ID: P37231
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Schlagworte: NR1C3, PPARG, PPAR-gamma, Nuclear receptor subfamily 1 group C member 3, Peroxisome proliferator-activated receptor gamma

96
wells

476 €

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Bemerkungen: Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the large family of nuclear hormone receptors. There are three subtypes, PPARalpha, beta/delta, and gamma, which are expressed at variable levels in different tissues. The Cayman Chemical PPARgamma Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPAR gamma binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE and PPARgamma is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
Image Art Cay10006855-96 Cayman PPARgamma Transcription Factor Assay Kit

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