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Cay10006855-96
PPARgamma Transcription Factor Assay Kit
Cayman
Icon Datasheet
96 wells

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476 €

PPARgamma Transcription Factor Assay Kit


PPARgamma binding detection Erklärungen der Abkürzungen ansehen.

Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the large family of nuclear hormone receptors. There are three subtypes, PPARalpha, beta/delta, and gamma, which are expressed at variable levels in different tissues. The Cayman Chemical PPARgamma Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPAR gamma binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE and PPARgamma is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.


UniProt ID: P37231

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Image Art 10006855 Cayman PPARgamma Transcription Factor Assay Kit


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Products for: NR1C3, PPARG - peroxisome proliferator-activated receptor gamma



Schlagworte:
NR1C3, PPARG, PPAR-​gamma, Nuclear receptor subfamily 1 group C member 3, Peroxisome proliferator-​activated receptor gamma

 


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