PPARalpha Transcription Factor Assay Kit
PPAR binding detection
Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the large family of nuclear hormone receptors. There are three subtypes, PPARalpha, beta/delta, and gamma, which are expressed at variable levels in different tissues. The Cayman Chemical PPAR.alpha. Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPARalpha binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE and PPARalpha is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm.
UniProt ID: Q07869
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Enzymes & Proteins
Products for: NR1C1, PPARA - peroxisome proliferator-activated receptor alpha
NR1C1, PPARA, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, Peroxisome proliferator-activated receptor alpha