PPARalpha Transcription Factor Assay Kit, Cayman, Cay10006915-96

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ART-NR	PRODUKT	HERSTELLER	MENGE	PREIS
Cay10006915-96	PPARalpha Transcription Factor Assay Kit	Cayman	96 wells Buy Bulk	476 €
PPAR binding detection Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors belonging to the large family of nuclear hormone receptors. There are three subtypes, PPARalpha, beta/delta, and gamma, which are expressed at variable levels in different tissues. The Cayman Chemical PPAR.alpha. Transcription Factor Assay is a non-radioactive, sensitive method for detecting PPARalpha binding activity in nuclear extracts. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double-stranded DNA (dsDNA) sequence containing the peroxisome proliferator response element (PPRE) is immobilized onto the wells of a 96-well plate. PPARs contained in a nuclear extract bind to the PPRE and PPARalpha is selectively detected using a specific primary antibody. A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. UniProt ID: Q07869 Loading ... Weitere Produkte für dieses Protein: Antibodies Anti-PPAR-alpha (Ab-21), Kat #: ABD-8B0975.50 Anti-PPARA (Center), Kat #: WA-AP12910c Anti-PPARa (Peroxisome Proliferator Activated Receptor, alpha), Kat #: P5505-07C3.50 Anti-PPARa (Peroxisome Proliferator Activated Receptor, alpha), Kat #: P5505-07C1.50 Anti-PPARa (Peroxisome Proliferator Activated Receptor, alpha), Kat #: P5505-07C2.50 Anti-PPARa (Peroxisome Proliferator Activated Receptor, alpha), Kat #: P5505-07C.50 Anti-PPARa (Peroxisome Proliferator Activated Receptor, alpha, HPPAR, MGC2237, MGC2452, NR1C1), Kat #: P5505-07E.50 Anti-PPARalpha, Kat #: Cay101710-500 Enzymes & Proteins PPAR alpha LBD (human recombinant), Kat #: Cay10009088-25 PPAR alpha LBD (human recombinant), Kat #: Cay10009088-50 PPAR alpha LBD (human recombinant), Kat #: Cay10009088-100 Transcription Factor Complete PPARalpha positive control, Kat #: Cay10008892-1 Transcription Factor PPARalpha Positive Control, Kat #: Cay10007441-1 Primers PPARA, Human peroxisome proliferator-activated receptor alpha, Real Time PCR Primer Set, Kat #: VHPS-7122 Relevant Pathways: PPAR signaling pathway Adipocytokine signaling pathway Hepatitis C Products for: NR1C1, PPARA - peroxisome proliferator-activated receptor alpha Antibodies (10) Chemicals & More (39) ELISA and Assay Kits (1) Enzymes & Proteins (5) Primers (3) Schlagworte: NR1C1, PPARA, PPAR-alpha, Nuclear receptor subfamily 1 group C member 1, Peroxisome proliferator-activated receptor alpha