PPARalpha Ligand-binding Domain (human, recombinant)

Peroxisome proliferator-activated receptor alpha (PPARalpha) is a member of the nuclear receptor family of ligand-activated transcription factors that regulates a variety of metabolic functions and inflammation. It contains an N-terminal domain that is subject to phosphorylation, a DNA-binding domain, and a C-terminal ligand-binding domain. PPARalpha is highly expressed in tissues with high fatty acid oxidation rates, including the liver, heart, skeletal muscle, brown adipose tissue, and kidney, as well as in macrophages and T cells. It is activated by a variety of endogenous ligands such as fatty acids, eicosanoids, and endocannabinoids, as well as synthetic agents, including fenofibrate (Cay-10005368) and gemfibrozil (Cay-14835). Upon activation, PPARalpha heterodimerizes with the retinoid X receptor (RXR) and binds to PPAR response elements in PPARalpha target genes, recruiting RNA polymerase II and initiating gene transcription. PPARalpha transcriptionally regulates a variety of genes involved in several cellular processes, including lipid and hormone transport, peroxisomal and mitochondrial beta-oxidation, amino acid metabolism, and inflammation. Genome-wide deletion of Ppara protects mice from high-fat diet-induced hyperinsulinemia and insulin resistance. Ppara SNPs have been found in individuals with a variety of cardiovascular conditions, including hypertension, atherosclerosis, coronary artery disease, left ventricular hypertrophy, or myocardial infarction. Formulations containing PPARalpha agonists have been used in the treatment of hyperlipidemia. Cayman's PPARalpha Ligand-binding Domain protein can be used for Western blot (WB) applications.Synonyms: Peroxisome Proliferator-activated Receptor alpha Ligand Binding Domain, PPARalpha LBD. Purity: >55%. Source: Recombinant human His-tagged PPARalpha ligand-binding domain expressed in E. coli. Amino Acids: 170-430. MW: ~34 kDa/subunit. Formulation: (Request formulation change), 50 mM sodium phosphate, pH 7.2, with 20% glycerol and 100 mM sodium chloride. UniProt Accession #: Q07869.