PDE8A Assay Kit

Phosphodiesterases (PDEs) play an important role in dynamic regulation of cAMP and cGMP signaling. PDE8 is involved in many biological processes, including T-cell activation, testosterone production, adrenocortical hyperplasia, and thyroid function. The PDE8A Assay Kit is designed for identification of PDE8A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE8A to the binding agent. The PDE8A inhibitor screening assay kit comes in a convenient 96-well format, including purified PDE8A1 enzyme, fluorescently labeled PDE8A substrate (cAMP), binding agent, and PDE assay buffer for 100 enzyme reactions. The key to the PDE8A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE8A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE8A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader.