NSD2 Chemiluminescent Assay Kit

The NSD2 Chemiluminescent Assay Kit is designed to measure NSD2 activity for screening and profiling applications. The NSD2 Chemiluminescent Assay Kit comes in a convenient format, with 96-well plate precoated with histone H3 substrate, the antibody against methylated lysine residue of Histone H3, a secondary HRP-labeled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified NSD2 enzyme for 96 enzyme reactions. The key to the NSD2 Chemiluminescent Activity Assay Kit is a highly specific antibody that recognizes methylated residue of Histone H3. With this kit, only three simple steps are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.