NF-kappaB (human p50) Transcription Factor Assay Kit

Cayman's NF-kappaB (human p50) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific transcription factor DNA binding activity in nuclear extracts and whole cell lysates. A 96-well enzyme-linked immunosorbent assay (ELISA) replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA). A specific double stranded DNA (dsDNA) sequence containing the NF-kappaB response element is immobilized to the wells of a 96-well plate. NF-kappaB contained in a nuclear extract, binds specifically to the NF-kappaB response element. NF-kappaB (p50) is detected by addition of specific primary antibody directed against NF-kappaB (p50). A secondary antibody conjugated to HRP is added to provide a sensitive colorimetric readout at 450 nm. The Cayman Chemical NF-kappaB (human p50) Transcription Factor Assay detects human NF-kappaB (p50). It will not cross-react with NF-kappaB (p65).Origin: Animal/Rabbit, Animal/Bovine, Animal/Goat.