Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.  Early attempts to cultivate normal mammalian fibroblasts and certain subtypes of HeLa cells revealed that they had specific nutritional requirements that could not be met by Eagle's Basal...

Used as a medium for cultivation of Mycobacterium sp. when supplemented with glycerol and OADC. Modification of Middlebrook 7H9 or 7H11 formulations. Expected Cultural Response (2-4 weeks at 35-37°C): Mycobacterium smegmatis ATCC 14468: Growth, Mycobacterium fortuitum ATCC 6841: Growth, Mycobacterium tuberculosis...