WNK [with no lysine (K)] family of serine/threonine kinases is characterized by having a cysteine in place of lysine in subdomain II of its kinase activation domain (1,2). The lysine necessary for phosphoryl transfer is located in an atypical position in the catalytic domain. Four WNK family members have been...

Ceftriaxone is a cephalosporin antibiotic. It is selectively active against Gram-negative bacteria, including N. gonorrhea and S. pyogenes (MICs = 0.01-0.025 and 0.012-0.25 µg/ml, respectively), over Gram-positive bacteria such as S. epidermidis and S. aureus (MICs = >25 µg/ml for both). Ceftriaxone inhibits S....

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....